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mouse stem cell factor  (R&D Systems)


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    Structured Review

    R&D Systems mouse stem cell factor
    Mouse Stem Cell Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+stem+cell+factor+scf/Recombinant+Mouse+SCF+Protein/pmc12999497-385-33-38
    Average 95 stars, based on 145 article reviews
    mouse stem cell factor - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Recombinant:

    Article Title: Secretion of Mast Cell Inflammatory Mediators Is Enhanced by CADM1-Dependent Adhesion to Sensory Neurons
    Article Snippet: For cell culture, complete medium was supplemented with 10% heat-inactivated fetal calf serum (FCS, Gibco, United Kingdom), 1% MEM Vitamin (Gibco, United Kingdom), 1% of sodium pyruvate (Gibco, United Kingdom), 100 IU/ml Penicillin, 100 μg/ml streptomycin (PAA Laboratories, United Kingdom), and 0.1 mM non-essential amino acid (Gibco, United Kingdom). .. In the final step, 10 ng/ml of recombinant mouse stem cell factor SCF (R&D systems, MN, United States) and 5 ng/ml recombinant murine IL-3 (R&D Systems, MN, United States) were added. ..

    Article Title: Type 3 secretion system induced leukotriene B4 synthesis by leukocytes is actively inhibited by Yersinia pestis to evade early immune recognition
    Article Snippet: .. Briefly, isolated bone marrow cells were resuspended in BMMC culture medium [DMEM containing 10% FCS, penicillin (100 units/mL), streptomycin (100 mg/mL), 2 mmol/L L-glutamine, and 50 mmol/L β-mercaptoethanol] supplemented with recombinant mouse stem cell factor (SCF) (12.5 ng/mL; R&D Systems Cat. No. 455-MC) and recombinant mouse IL-3 (10 ng/mL; R&D Systems Cat. No. 403-ML). ..

    Article Title: An in vitro approach reveals molecular mechanisms underlying endocrine disruptor-induced epimutagenesis
    Article Snippet: Chemical compound , Recombinant human bone morphogenetic protein 4 (BMP-4) , R&D Systems , 314 BP-010 , . .. Chemical compound , Recombinant mouse stem cell factor (SCF) , R&D Systems , 455-MC-010 , . .. Chemical compound , Recombinant human epidermal growth factor (EGF), carrier free (CF) , R&D Systems , 2028-EG-200 , .

    Article Title: Extracellular ATP is involved in the induction of apoptosis in murine hematopoietic cells.
    Article Snippet: .. Recombinant mouse stem cell factor (SCF) and IL-6, purchased from R&D Systems, were used for sustaining cells in vitro. .. ATP was purchased from Sigma.

    Article Title: Inhibition of type III secretion system induced leukotriene B 4 production by Yersinia pestis : A mechanism for early immune evasion
    Article Snippet: .. Briefly, isolated bone marrow cells were resuspended in BMMC culture medium [DMEM containing 10% FCS, penicillin (100 units/mL), streptomycin (100 mg/mL), 2 mmol/L L-glutamine, and 50 mmol/L β-mercaptoethanol] supplemented with recombinant mouse stem cell factor (SCF) (12.5 ng/mL; R&D Systems, Cat. No. 455-MC) and recombinant mouse IL-3 (10 ng/mL; R&D Systems, Cat. No. 403-ML). ..

    Article Title: Gene-Centric Functional Dissection of Human Genetic Variation Uncovers Regulators of Hematopoiesis
    Article Snippet: .. The cells were cultured in erythroid maintenance medium (StemSpan-SFEM; StemCell Technologies) supplemented with 100 ng/mL recombinant mouse stem cell factor (SCF) (R&D Systems), 40 ng/mL recombinant mouse IGF1 (R&D Systems), 100 nM dexamethasone (Sigma), and 2 U/mL erythropoietin (Amgen) and cultured at 37°C for 36 hours. .. Following this the cells were cultured for another 48 hours in erythroid differentiation medium (Iscove modified Dulbecco’s medium containing 15% (vol/vol) FBS (Stemcell), 1% detoxified BSA (Stemcell), 500 μg/mL holo-transferrin (Sigma-Aldrich), 0.5 U/mL Epoetin (Epo; Amgen), 10 μg/mL recombinant human insulin (Sigma-Aldrich), and 2 mM L-glutamine (Invitrogen)) at 37 °C.

    Article Title: Gene-centric functional dissection of human genetic variation uncovers regulators of hematopoiesis
    Article Snippet: .. The cells were cultured in erythroid maintenance medium (StemSpan-SFEM; StemCell Technologies) supplemented with 100 ng/mL recombinant mouse stem cell factor (SCF) (R&D Systems), 40 ng/mL recombinant mouse IGF1 (R&D Systems), 100 nM dexamethasone (Sigma), and 2 U/mL erythropoietin (Amgen) and cultured at 37°C for 36 hr. .. Following this, the cells were cultured for another 48 hr in erythroid differentiation medium (Iscove modified Dulbecco’s medium containing 15% (vol/vol) FBS (Stemcell), 1% detoxified BSA (Stemcell), 500 μg/mL holo-transferrin (Sigma-Aldrich), 0.5 U/mL Epoetin (Epo; Amgen), 10 μg/mL recombinant human insulin (Sigma-Aldrich), and 2 mM L-glutamine (Invitrogen)) at 37°C.

    Article Title: Gene-centric functional dissection of human genetic variation uncovers regulators of hematopoiesis
    Article Snippet: .. The cells were cultured in erythroid maintenance medium (StemSpan-SFEM; StemCell Technologies) supplemented with 100 ng/ mL recombinant mouse stem cell factor (SCF) (R&D Systems), 40 ng/mL recombinant mouse IGF1 (R&D Systems), 100 nM dexamethasone (Sigma), and 2 U/mL erythropoietin (Amgen) and cultured at 37 ̊C for 36 hr. .. Following this, the cells were cultured for another 48 hr in erythroid differentiation medium (Iscove modified Dulbecco’s medium containing 15% (vol/vol) FBS (Stemcell), 1% detoxified BSA (Stemcell), 500 mg/mL holo-transferrin (Sigma-Aldrich), 0.5 U/mL Epoetin (Epo; Amgen), 10 mg/ mL recombinant human insulin (Sigma-Aldrich), and 2 mM L-glutamine (Invitrogen)) at 37 ̊C.

    Isolation:

    Article Title: Type 3 secretion system induced leukotriene B4 synthesis by leukocytes is actively inhibited by Yersinia pestis to evade early immune recognition
    Article Snippet: .. Briefly, isolated bone marrow cells were resuspended in BMMC culture medium [DMEM containing 10% FCS, penicillin (100 units/mL), streptomycin (100 mg/mL), 2 mmol/L L-glutamine, and 50 mmol/L β-mercaptoethanol] supplemented with recombinant mouse stem cell factor (SCF) (12.5 ng/mL; R&D Systems Cat. No. 455-MC) and recombinant mouse IL-3 (10 ng/mL; R&D Systems Cat. No. 403-ML). ..

    Article Title: Inhibition of type III secretion system induced leukotriene B 4 production by Yersinia pestis : A mechanism for early immune evasion
    Article Snippet: .. Briefly, isolated bone marrow cells were resuspended in BMMC culture medium [DMEM containing 10% FCS, penicillin (100 units/mL), streptomycin (100 mg/mL), 2 mmol/L L-glutamine, and 50 mmol/L β-mercaptoethanol] supplemented with recombinant mouse stem cell factor (SCF) (12.5 ng/mL; R&D Systems, Cat. No. 455-MC) and recombinant mouse IL-3 (10 ng/mL; R&D Systems, Cat. No. 403-ML). ..

    In Vitro:

    Article Title: Extracellular ATP is involved in the induction of apoptosis in murine hematopoietic cells.
    Article Snippet: .. Recombinant mouse stem cell factor (SCF) and IL-6, purchased from R&D Systems, were used for sustaining cells in vitro. .. ATP was purchased from Sigma.

    Cell Culture:

    Article Title: Gene-Centric Functional Dissection of Human Genetic Variation Uncovers Regulators of Hematopoiesis
    Article Snippet: .. The cells were cultured in erythroid maintenance medium (StemSpan-SFEM; StemCell Technologies) supplemented with 100 ng/mL recombinant mouse stem cell factor (SCF) (R&D Systems), 40 ng/mL recombinant mouse IGF1 (R&D Systems), 100 nM dexamethasone (Sigma), and 2 U/mL erythropoietin (Amgen) and cultured at 37°C for 36 hours. .. Following this the cells were cultured for another 48 hours in erythroid differentiation medium (Iscove modified Dulbecco’s medium containing 15% (vol/vol) FBS (Stemcell), 1% detoxified BSA (Stemcell), 500 μg/mL holo-transferrin (Sigma-Aldrich), 0.5 U/mL Epoetin (Epo; Amgen), 10 μg/mL recombinant human insulin (Sigma-Aldrich), and 2 mM L-glutamine (Invitrogen)) at 37 °C.

    Article Title: Gene-centric functional dissection of human genetic variation uncovers regulators of hematopoiesis
    Article Snippet: .. The cells were cultured in erythroid maintenance medium (StemSpan-SFEM; StemCell Technologies) supplemented with 100 ng/mL recombinant mouse stem cell factor (SCF) (R&D Systems), 40 ng/mL recombinant mouse IGF1 (R&D Systems), 100 nM dexamethasone (Sigma), and 2 U/mL erythropoietin (Amgen) and cultured at 37°C for 36 hr. .. Following this, the cells were cultured for another 48 hr in erythroid differentiation medium (Iscove modified Dulbecco’s medium containing 15% (vol/vol) FBS (Stemcell), 1% detoxified BSA (Stemcell), 500 μg/mL holo-transferrin (Sigma-Aldrich), 0.5 U/mL Epoetin (Epo; Amgen), 10 μg/mL recombinant human insulin (Sigma-Aldrich), and 2 mM L-glutamine (Invitrogen)) at 37°C.

    Article Title: Gene-centric functional dissection of human genetic variation uncovers regulators of hematopoiesis
    Article Snippet: .. The cells were cultured in erythroid maintenance medium (StemSpan-SFEM; StemCell Technologies) supplemented with 100 ng/ mL recombinant mouse stem cell factor (SCF) (R&D Systems), 40 ng/mL recombinant mouse IGF1 (R&D Systems), 100 nM dexamethasone (Sigma), and 2 U/mL erythropoietin (Amgen) and cultured at 37 ̊C for 36 hr. .. Following this, the cells were cultured for another 48 hr in erythroid differentiation medium (Iscove modified Dulbecco’s medium containing 15% (vol/vol) FBS (Stemcell), 1% detoxified BSA (Stemcell), 500 mg/mL holo-transferrin (Sigma-Aldrich), 0.5 U/mL Epoetin (Epo; Amgen), 10 mg/ mL recombinant human insulin (Sigma-Aldrich), and 2 mM L-glutamine (Invitrogen)) at 37 ̊C.



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